Developmental expression of GnRH3:EMD in terminal nerve, 24 - 31 hours post-fertilization (hpf); confocal microscopy timelapse:

Bilateral clusters of terminal nerve GnRH3 neurons send projections towards each other, potentially making contact; confocal microscopy 3-D reconstruction:

Close apposition of projections from GnRH3 neurons from terminal nerve and preoptic area populations; confocal microscopy 3-D reconstruction:

Developmental expression of GnRH3:EMD in trigeminal neurons and spinal cord, 17 - 24 hpf; confocal microscopy timelapse:

Close apposition of projections from GnRH3:EMD expressing neurons in the trigeminal and spinal cord populations; confocal microscopy 3-D reconstruction:

Developmental expression of GnRH3:EMD throughout the head and eyes, 23-45 hpf; confocal microscopy timelapse: