Dr. Ping's current research program focuses on the understanding of proteome biology in cardiovascular medicine,
with a particular interest on alterations of subproteomes encoding signaling pathways and cellular organelles during
cardiac pathogenesis. Her research group investigates specific areas in:
Mitochondrial Biology in the Heart:
Cardiac mitochondria proteome and phosphoproteome: The subproteome composition and post-translational
modification profiles define the functional state of the cardiac mitochondria. My group employs state-of-the-art
mass spectrometry expertise to interrogate their dynamics in health and diseases. Mitochondrial proteome turnover:
The body of mitochondrial proteome is continuously replaced. Such kinetics varies greatly among distinct proteins
and even for the same protein under different pathophysiological states. A novel strategy to systematically characterize
their perturbations has been devised in our research group. Mitochondrial permeability transition (MPT): MPT is caused by
the opening of permeability transition pores in the inner mitochondrial membrane and may lead to cell death.
A multi-disciplinary approach using an array of omics tools is applied to characterize MPT regulation
(i.e., their interacting partners) in the healthy and diseased myocardium.
Proteasome Biology in the Heart:
Proteasome function: Our research group has established necessary toolboxes for the investigation of
ubiquitin-proteasome-dependent degradation of proteins in the heart. We combine both biochemical and proteomic
technologies to investigate 20S and 26S proteasome complexes in the normal, protected, and diseased heart.
Proteasome heterogeneity: A heterogenic population of proteasome complexes exists in the heart, the composition
of which is subject to change upon stress. A powerful technical platform has been assembled to characterize each
subpopulation individually with its native constituents, which enables us to pinpoint specific changes.
Proteasome regulation by post-translational modifications: The functional dynamics of the proteasome complexes are
regulated via various forms of post-translational modifications.
Cardiac Organellar Protein atlas Knowledgebase (COPaKB):
We have developed a COPa knowledgebase, a specialized
resource for cardiac proteome biology. It integrates orthogonal sets of proteome knowledge and biomedical insights into
context, while providing bioinformatics tools and web portals to efficiently disseminate proteomics proficiencies.
It bridges data-driven proteomic discoveries and hypothesis-driven investigations, facilitates synergistic research
paradigm across the research community, thereby advancing cardiovascular biology and medicine.
Deng N, Zhang J, Zong C, Wang Y, Lu H, Yang P, Wang W, Young GW, Wang Y, Korge P, Lotz, C, Doran, P , Liem, DA, Apweiler R, Weiss JN, Duan H, Ping P. (2011) Phosphoproteome Analysis Reveals Regulatory Sites in Major pathways of Cardiac Mitochondria. Mol Cell Proteomics. 10:M110.000117
Ping P. Getting to the Heart of Proteomics. (2009) New Eng J Med. 360: 532-534.
Zong C, Gomes AV, Drews O, Li X, Young GW, Berhane B, Qiao X, French SW, Bardag-Gorce F, Ping P. (2006) Regulation of Murine Cardiac 20S Proteasomes: Role of Associating Partners. Circ Res. 99:372-380.