Dr. H. Ronald Kaback
Structural Studies & Biophysics
6-720 Macdonal Research Laboratories
310-206-5053
rkaback@mednet.ucla.edu

Selected Publications:

Kaback, H.R., Dunten, R., Frillingos, S., Venkatesan, P., Kwaw, I., Zhang, W. & Ermolova, N. 2007. Site-directed alkylation and the alternating access model for LacY. Proc Natl Acad Sci USA 104, 491-4.

Majumdar, D. S., Smirnova, I., Kasho, V., Nir, E., Kong, X., Weiss, S. & Kaback, H.R. 2007. Single-molecule FRET reveals sugar-induced conformational dynamics in LacY. Proc Natl Acad Sci USA 104, 12640-5.

Shimohata, N., Nagamori, S., Akiyama, Y., Kaback, H.R. & Ito, K. 2007. J Cell Biol. 176, 307-17.

Guan, L. & Kaback, H.R. 2007. Site-directed alkylation of cysteine to test solvent accessibility of membrane proteins. Nature Protocols. 2, 2012-7.

Guan, L., Mirza, O., Verner, G., Iwata, S. & Kaback, H.R. 2007. Structural determination of wild-type lactose permease. Proc Natl Acad Sci USA 104, 15294-8.

Smirnova, I., Kasho, V., Choe, J.-Y., Altenbach, C., Hubbell, W. L. & Kaback, H.R. 2007. Sugar binding induces an outward facing conformation of LacY. Proc Natl Acad Sci USA 104, 16504-9.

Nie, Y., Ermolova, N. & Kaback, H.R. 2007. Site-directed alkylation of LacY: effect of the proton electrochemical gradient. J Mol Biol 374, 356-64.

Zhou Y., Guan L., Freites J.A., Kaback, H.R. 2008. Opening and closing of the periplasmic gate in lactose permease. Proc Natl Acad Sci 105, 3774-8.

Dong Y., Palmer S.R., Hasona A., Nagamori S., Kaback, H.R., Dalbey R.E., Brady L.J. 2008. Functional overlap but lack of complete cross-complementation of Streptococcus mutants and Escherichia coli YidC orthologs. J Bacteriol 190, 2458-69.

Nie Y., Sabetford F.E., Kaback, H.R. 2008. The Cys154→Gly mutation in LacY causes constitutive opening of the hydrophilic periplasmic pathway. J Mol Biol 379, 695-703.

Smirnova IN, Kasho V, Kaback, H.R. Protonation and sugar binding to LacY. Proc Natl Acad Sci U S A. 2008 Jul 1;105(26):8896-901

Nie, Y., Zhou, Y & Kaback, H.R. 2009. Clogging the Periplasmic Pathway in LacY. Biochemistry, 48, 738-743.

Garcia-Celma, JJ, Smirnova, I., Kaback, H.R., Altenbach, Fendler, K. 2009. Electrophysiological characterization of LacY. Proc Natl Acad Sci USA 106, 7373-78.

Smirnova I, Kasho V, Sugihara J, Choe JY, Kaback, H.R. Residues in the H+ translocation site define the pKa for sugar binding to LacY. Biochemistry. 2009 Sep 22;48(37):8852-60.