Astrocyte RNA-Seq

• • Adult Astrocyte RNA-Seq Explorer
• • Adult Astrocyte RNA-Seq Explorer - Huntington Data

Tools to study astrocytes

• P2X4-tdTomato BAC mice, GAT2-tdTomato BAC mice, astrocyte specific Cre/ERT2 mouse lines, Rosa26 lox-STOP-lox-Lck-GCaMP6f mice, wearable microscopes

• Genetically encoded calcium indicator (GECI) plasmids for astrocytes and AAVs (e.g. Lck-GCaMP3, Lck-GCaMP6, tdTomato)
http://www.addgene.org/Baljit_Khakh/

• AAV2/5 viruses (e.g. AAV GfaABC1D promoter driven GCaMP3, GCaMP6, Lck-GCaMP3, Lck-GCaMP6f, tdTomato, Lck-GFP, R-GECO, Kir4.1-GFP, MrgA1-mCherry)
http://www.med.upenn.edu/gtp/vectorcore/

• SPRAE mice to drive Mossy fiber neurotransmitter release (JAX Stock No. 025114)
http://jaxmice.jax.org/

• GECIquant software and Java code to analyse calcium signals.
http://www.ncbi.nlm.nih.gov/pubmed/25894291
GECIquant manual, code and plugin for ImageJ or Fiji

• Protocols for preparing hippocampal neurons and astrocytes, and for performing TIRF microscopy
http://www.jove.com/video/1142/measuring-near-plasma-membrane-global-intracellular-calcium-dynamics

• Protocols for GECI expression within astrocytes in vivo
http://www.ncbi.nlm.nih.gov/pubmed/23589582

• Protocol for in vivo microinjections of AAV
http://www.ncbi.nlm.nih.gov/pubmed/25490346

• Cre-dependent knock-in Lck-GCaMP6f mice at the ROSA26 locus
https://www.jax.org/strain/029626

• Aldh1l1-Cre/ERT2 mice
https://www.jax.org/strain/029655

• Slc6a11-Cre/ERT2 mice
https://www.jax.org/strain/029656

Tools to study P2X ion channels

• Imaging P2X4 expressing cells with novel reporter mice
https://www.jax.org/strain/029332
https://www.ncbi.nlm.nih.gov/pubmed/27559172

• Approach to image P2X trafficking
http://www.ncbi.nlm.nih.gov/pubmed/24935743

• Tracking P2X channel activation with FRET
http://www.ncbi.nlm.nih.gov/pubmed/18084300

• Approach to image single P2X channels
http://www.ncbi.nlm.nih.gov/pubmed/22090499